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KMID : 1140920190430030328
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Annals of Rehabilitation Medicine
2019 Volume.43 No. 3 p.328 ~ p.334
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The Value of MicroRNAs as an Indicator of the Severity and the Acute Phase of Spinal Cord Injury
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Park Jun-Young
Yi Dong-Soo
Jang Ji-Yoon
Hong Ji-Seon
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Abstract
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Objective: To assess the role of miRNA-21 and miRNA-223 in a balloon-compression model of spinal cord injury (SCI).
Methods: A total of 50 male Wistar rats (n=50) were divided into the three groups: the group A (n=15, insertion of the unflated Fogarty balloon catheter), the group B (n=15, insertion of the Fogarty balloon catheter at a volume of 20 μL) and the group C (n=15, insertion of the Fogarty balloon catheter at a volume of 50 μL). After the behavioral test, RNA isolation, microRNA expression profiling using microarrays and quantitative polymerase chain reaction, measurements were compared between the three groups.
Results: Despite a lack of significant differences in time-dependent changes in miRNA-21 expression levels between the three groups at 4 hours, there were significant differences in them at 1, 3, and 7 days (p<0.05). Moreover, there were significant differences in time-dependent changes in miRNA-223 expression levels between the three groups at 4 hours and 1, 3, and 7 days (p<0.05). Furthermore, miRNA-223 expression levels reached the highest at 1 day but were decreased with time thereafter in all the three groups.
Conclusion: Expression levels of miRNA-21 and miRNA-223 might be associated with the severity and acute phase of SCI, respectively. It is mandatory, however, to analyze changes in levels of inflammatory markers and the relevant biological pathways.
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KEYWORD
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Spinal cord, Spinal cord injuries, MicroRNAs, Gene expression regulation, Therapeutics
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